Open AccessRecent advances in fluorescence-based in vivo flow cytometry
Author(s) -
K. Sandy Pang,
Bobo Gu,
Feng Liu,
Mingli Dong,
Lianqing Zhu,
Xunbin Wei
Publication year - 2019
Publication title -
journal of innovative optical health sciences/journal of innovation in optical health science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.421
H-Index - 24
eISSN - 1793-5458
pISSN - 1793-7205
DOI - 10.1142/s1793545819300088
Subject(s) - flow cytometry , multispectral image , in vivo , photoacoustic imaging in biomedicine , fluorescence , cytometry , preclinical imaging , fluorescence lifetime imaging microscopy , computer science , biomedical engineering , nanotechnology , materials science , medicine , biology , optics , microbiology and biotechnology , physics , artificial intelligence
The fluorescence-based in vivo flow cytometry (IVFC) is an emerging tool to monitor circulating cells in vivo. As a noninvasive and real-time diagnostic technology, the fluorescence-based IVFC allows long-term monitoring of circulating cells without changing their native biological environment. It has been applied for various biological applications (e.g., monitoring circulating tumor cells). In this work, we will review our recent works on fluorescence-based IVFC. The operation principle and typical biological applications will be introduced. In addition, the recent advances in IVFC flow cytometry based on photoacoustic effects and other label-free detection methods such as imaging-based methods, diffuse-light methods, hybrid multimodality methods and multispectral methods are also summarized.