Open AccessStructured illumination microscopy and its new developments
Author(s) -
Jianling Chen,
Caimin Qiu,
Minghai You,
Xiaogang Chen,
Hongqin Yang,
Shusen Xie
Publication year - 2016
Publication title -
journal of innovative optical health sciences/journal of innovation in optical health science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.421
H-Index - 24
eISSN - 1793-5458
pISSN - 1793-7205
DOI - 10.1142/s179354581630010x
Subject(s) - microscopy , resolution (logic) , optics , optical microscope , light sheet fluorescence microscopy , super resolution microscopy , diffraction , image resolution , materials science , photoactivated localization microscopy , nanotechnology , scanning confocal electron microscopy , physics , computer science , artificial intelligence , scanning electron microscope
Optical microscopy allows us to observe the biological structures and processes within living cells. However, the spatial resolution of the optical microscopy is limited to about half of the wavelength by the light diffraction. Structured illumination microscopy (SIM), a type of new emerging super-resolution microscopy, doubles the spatial resolution by illuminating the specimen with a patterned light, and the sample and light source requirements of SIM are not as strict as the other super-resolution microscopy. In addition, SIM is easier to combine with the other imaging techniques to improve their imaging resolution, leading to the developments of diverse types of SIM. SIM has great potential to meet the various requirements of living cells imaging. Here, we review the recent developments of SIM and its combination with other imaging techniques