Open AccessCOMPARISON OF MACROMOLECULAR COMPONENT DISTRIBUTIONS IN OSTEOARTHRITIC AND HEALTHY CARTILAGES BY FOURIER TRANSFORM INFRARED IMAGING
Author(s) -
Jianhua Yin,
Yang Xia,
Zhiliang Xiao
Publication year - 2013
Publication title -
journal of innovative optical health sciences/journal of innovation in optical health science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.421
H-Index - 24
eISSN - 1793-5458
pISSN - 1793-7205
DOI - 10.1142/s179354581350048x
Subject(s) - proteoglycan , cartilage , osteoarthritis , infrared , fourier transform infrared spectroscopy , chemistry , fourier transform , pathological , anatomy , pathology , biophysics , nuclear magnetic resonance , biomedical engineering , materials science , medicine , biology , optics , mathematical analysis , physics , alternative medicine , mathematics
Fourier transform infrared imaging (FTIRI) was used to examine the depth-dependent content variations of macromolecular components, collagen and proteoglycan (PG), in osteoarthritic and healthy cartilages. Dried 6 μm thick sections of canine knee cartilages were imaged at 6.25 μm pixel-size in FTIRI. By analyzing the infrared (IR) images and spectra, the depth dependence of characteristic band (sugar) intensity of PG show obvious difference between the cartilage sections of (OA) and health. The result confirms that PG content decreases in the osteoarthritic cartilage. However, no clear change occurs to collagen, suggesting that the OA influences little on the collagen content at early stage of OA. This observation will be helpful to further understand PG loss associated with pathological conditions in OA, and demonstrates that FTIRI has the potential to become an important analytical tool to identify early clinical signs of tissue degradation, such as PG loss even collagen disruption