Open AccessMULTIPHOTON MICROSCOPIC IMAGING OF MOUSE INTESTINAL MUCOSA BASED ON TWO-PHOTON EXCITED FLUORESCENCE AND SECOND HARMONIC GENERATION
Author(s) -
Ren'an Xu,
Xiaodong Zhu,
Ning He,
Shuangmu Zhuo,
Jian Xu,
Shanghai Jiang,
Hongsheng Li,
Jianxin Chen
Publication year - 2013
Publication title -
journal of innovative optical health sciences/journal of innovation in optical health science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.421
H-Index - 24
eISSN - 1793-5458
pISSN - 1793-7205
DOI - 10.1142/s1793545813500041
Subject(s) - fluorescence , two photon excitation microscopy , pathology , microscopy , multiphoton fluorescence microscope , second harmonic generation , chemistry , in vivo , intestinal mucosa , fluorescence microscope , biophysics , materials science , optics , biology , medicine , laser , physics , microbiology and biotechnology
Multiphoton microscopy (MPM), based on two-photon excited fluorescence and second harmonic generation, enables direct noninvasive visualization of tissue architecture and cell morphology in live tissues without the administration of exogenous contrast agents. In this paper, we used MPM to image the microstructures of the mucosa in fresh, unfixed, and unstained intestinal tissue of mouse. The morphology and distribution of the main components in mucosa layer such as columnar cells, goblet cells, intestinal glands, and a little collagen fibers were clearly observed in MPM images, and then compared with standard H&E images from paired specimens. Our results indicate that MPM combined with endoscopy and miniaturization probes has the potential application in the clinical diagnosis and in vivo monitoring of early intestinal cancer