Open AccessInduction and repair of DNA strand breaks in Bacteroides fragilis
Author(s) -
Valerie R. Abratt,
Meyrick J. Peak,
Jennifer G. Peak,
Joseph D. Santangelo,
David R. Woods
Publication year - 1990
Publication title -
canadian journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.635
H-Index - 94
eISSN - 1480-3275
pISSN - 0008-4166
DOI - 10.1139/m90-085
Subject(s) - bacteroides fragilis , mutant , breakage , mitomycin c , dna , biology , dna repair , wild type , microbiology and biotechnology , chemistry , biochemistry , genetics , antibiotics , materials science , composite material , gene
Alkaline sucrose gradient sedimentation was used to establish whether strand breakage and repair take place in the DNA of UV-irradiated Bacteroides fragilis during the removal of pyrimidine dimers. A B. fragilis wild-type strain and two of its repair mutants, a mitomycin C sensitive mutant (MTC25) having wild-type levels of UV survival, and a UV-sensitive, mitomycin C sensitive mutant (UVS9), were investigated. Under anaerobic conditions, far-UV irradiation induced metabolically regulated strand breakage and resynthesis in the wild-type strain, but this was markedly reduced in both the MTC25 and UVS9 mutants. Approximately half of the strand breaks generated by the various strains were rejoined during further holding in buffer. Under replicating conditions, complete repair of strand breaks in the wild type was observed. Caffeine treatment under anaerobic conditions caused direct DNA strand breakage in B. fragilis cells but did not inhibit UV-induced breakage or repair.
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