Open AccessValidation of a SNP-KASP marker for the Fusarium head blight resistance quantitative trait loci on chromosome 5AS
Author(s) -
Sudhakar Pandurangan,
Kirby T. Nilsen,
Santosh Kumar
Publication year - 2021
Publication title -
canadian journal of plant science/canadian journal of plant science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.338
H-Index - 59
eISSN - 1918-1833
pISSN - 0008-4220
DOI - 10.1139/cjps-2020-0099
Subject(s) - biology , quantitative trait locus , genetics , single nucleotide polymorphism , locus (genetics) , introgression , plant disease resistance , variants of pcr , fusarium , marker assisted selection , snp , genetic marker , microsatellite , polymerase chain reaction , allele , genotype , gene
Fusarium head blight (FHB) is a devastating wheat disease with a significant economic impact. Fhb5 is an important quantitative trait loci (QTL) conferring type I resistance to FHB. In this study, we accessed the usability of a Kompetitive allele-specific polymerase chain reaction (KASP) marker for the QTL on Fhb-5AS. The KASP clustering results were compared with the linked simple sequence repeat marker, wmc705 for Qfhb-5AS. Our results indicate that the single-nucleotide polymorphic locus wsnp_Ra_c24707_34262900 (IWA7777) provides reliable information for Qfhb-5AS-based resistance and would be amenable to masker-assisted selection, introgression of the resistance loci, and pyramiding of FHB resistance in wheat cultivars.