Open AccessThe bioactive lipid (S)-sebastenoic acid impacts motility and dispersion in Vibrio cholerae
Author(s) -
Christopher J. A. Warner,
Mauro Salinas,
David ZamoranoSánchez,
Walter M. Bray,
R. Scott Lokey,
Fitnat H. Yildiz,
Roger G. Linington
Publication year - 2018
Publication title -
canadian journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.323
H-Index - 68
eISSN - 1480-3291
pISSN - 0008-4042
DOI - 10.1139/cjc-2017-0334
Subject(s) - vibrio cholerae , biofilm , motility , virulence , chemistry , microbiology and biotechnology , regulator , cholera , bacteria , biology , biochemistry , gene , genetics
Although Gram-negative bacterial pathogens continue to impart a substantial burden on global healthcare systems, much remains to be understood about aspects of basic physiology in these organisms. In recent years, cyclic-diguanylate (c-di-GMP) has emerged as a key regulator of a number of important processes related to pathogenicity, including biofilm formation, motility and virulence. In an effort to discover chemical genetic probes for studying V. cholerae we have developed a new motility-based high-throughput screen to identify compounds that modulate c-di-GMP levels. Using this new screening platform, we tested a library of microbially-derived marine natural products extracts, leading to the discovery of the bioactive lipid (S)-sebastenoic acid. Evaluation of the effect of this new compound on bacterial motility, vpsL expression and biofilm formation implied that (S)-sebastenoic acid may alter phenotypes associated to c-di-GMP signaling in V. cholerae .