Diagnosis of botulism types C and D in cattle by a monoclonal antibody‐based sandwich ELISA | Zendy

Brooks C. E. | Zendy; Clarke H. J. | Zendy; Graham D. A. | Zendy; Ball H. J. | Zendy

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Diagnosis of botulism types C and D in cattle by a monoclonal antibody‐based sandwich ELISA

Author(s) -

Brooks C. E.,

Clarke H. J.,

Graham D. A.,

Ball H. J.

Publication year - 2011

Publication title -

veterinary record

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.261

H-Index - 99

eISSN - 2042-7670

pISSN - 0042-4900

DOI - 10.1136/vr.c7432

Subject(s) - botulism , monoclonal antibody , bioassay , toxin , suspect , clostridium botulinum , biology , microbiology and biotechnology , virology , antibody , vial , monoclonal , chemistry , immunology , chromatography , genetics , political science , law

This study was undertaken to evaluate two monoclonal antibody‐based sandwich ELISAs (sELISAs) for the detection of Clostridium botulinum neurotoxins (BoNTs) types C and D from culture‐enriched intestinal content samples from cattle. To validate the diagnostic significance of the presence of cultivable, toxin‐producing C botulinum in the intestines of cattle, samples from both suspect and non‐suspect botulism cases were examined. BoNT was detected by both sELISAs in a greater number of suspect animals than by direct testing of uncultured samples by mouse bioassay. One sELISA detected two BoNT C and one BoNT Group III mosaic isoform in three animals that were missed by the other, and both sELISAs failed to identify samples from two mouse bioassay‐positive BoNT C animals. BoNT D was also detected in one non‐suspect sample by one of the sELISAs.

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