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Comparison of tuberculin activity using the interferon‐γ assay for the diagnosis of bovine tuberculosis
Author(s) -
Schiller I.,
Vordermeier H. M.,
Waters W. R.,
Kyburz A.,
Cagiola M.,
Whelan A.,
Palmer M. V.,
Thacker T. C.,
Meijlis J.,
Carter C.,
Gordon S.,
Egnuni T.,
Hardegger R.,
MargHaufe B.,
Raeber A.,
Oesch B.
Publication year - 2010
Publication title -
veterinary record
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.261
H-Index - 99
eISSN - 2042-7670
pISSN - 0042-4900
DOI - 10.1136/vr.c3403
Subject(s) - tuberculin , bovine tuberculosis , tuberculosis , tuberculin test , interferon , interferon γ , virology , immunology , medicine , biology , mycobacterium bovis , mycobacterium tuberculosis , interferon gamma , pathology , immune system
In this study, interferon‐γ (IFN‐γ) responses in whole blood cultures stimulated with tuberculins from different sources were compared with regard to their diagnostic reliability in cattle experimentally and naturally infected with Mycobacterium bovis . The IFN‐γ responses to different concentrations of purified protein derivatives (PPDs) from M bovis and Mycobacterium avium were quantified. Significant differences (P<0.05) between sources and concentrations of PPDs used for stimulation were detected, indicating a need for standardisation of PPDs used in the IFN‐γ assay. Additionally, a tool named ‘relative potency 30‘ that allows rapid comparison of batches and sources of PPDs was defined.