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PCR survey of vectorborne pathogens in dogs living in and around Barcelona, an area endemic for leishmaniosis
Author(s) -
Tabar MD.,
Francino O.,
Altet L.,
Sánchez A.,
Ferrer L.,
Roura X.
Publication year - 2009
Publication title -
veterinary record
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.261
H-Index - 99
eISSN - 2042-7670
pISSN - 0042-4900
DOI - 10.1136/vr.164.4.112
Subject(s) - ehrlichia canis , leishmania infantum , babesia , ehrlichia , babesia canis , biology , hepatozoon , babesiosis , veterinary medicine , canis , theileria , virology , anaplasma , bartonella , anaplasma phagocytophilum , tick , medicine , parasite hosting , leishmaniasis , immunology , serology , visceral leishmaniasis , antibody , ecology , world wide web , computer science , borrelia burgdorferi
Blood samples from 153 dogs living in and around Barcelona were assayed for Leishmania infantum and Ehrlichia, Anaplasma, Rickettsia, Bartonella, Hepatozoon, Babesia and Theileria species by PCR amplification of DNA, and the amplicons obtained were sequenced. The prevalence of the infectious agents was L infantum (29·4 per cent), Ehrlichia and Anaplasma species (4·0 per cent), Hepatozoon canis (3·3 per cent), Babesia canis vogeli (2·0 per cent), Babesia gibsoni (2·0 per cent), Babesia canis canis (1·3 per cent) and Theileria annae (0·7 per cent). Coinfections were present in seven of the dogs and they were significantly associated with L infantum infection (P=0·024). There was a significant correlation between clinical signs of illness and the load of L infantum .