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Secondary immune‐mediated thrombocytopenia in dogs naturally infected by Leishmania infantum
Author(s) -
Cortese L.,
Piantedosi D.,
Ciaramella P.,
Pero M. E.,
Sica M.,
Ruggiero G.,
Terrazzano G.,
Mastellone V.
Publication year - 2009
Publication title -
veterinary record
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.261
H-Index - 99
eISSN - 2042-7670
pISSN - 0042-4900
DOI - 10.1136/vr.164.25.778
Subject(s) - leishmania infantum , platelet , antibody , immunology , immune system , flow cytometry , medicine , thrombocytopenic purpura , leishmania , immune thrombocytopenia , canine leishmaniasis , visceral leishmaniasis , leishmaniasis , parasite hosting , world wide web , computer science
Forty‐four dogs naturally infected by Leishmania infantum were divided into two groups: 20 thrombocytopenic dogs with fewer than 150 × 10 9 platelets/I, and 24 non‐thrombocytopenic dogs with more than 200 × 10 9 platelets/I. Ten clinically healthy dogs were used as controls. A haematological profile was obtained and the dogs' serum was used to assess the presence of platelet‐binding IgM and IgG antibodies using a flow cytometry technique. Nineteen of the 20 thrombocytopenic dogs, and 13 of the 24 non‐thrombocytopenic dogs had detectable levels of platelet‐binding immunoglobulins, but none of the control dogs did so. The differences were significantly different for both IgM and IgG platelet‐binding antibodies.