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Molecular characterisation of Mycobacterium bovis isolates from cattle carcases at a city slaughterhouse in Uganda
Author(s) -
Asiimwe B. B.,
Asiimwe J.,
Kallenius G.,
Ashaba F. K.,
Ghebremichael S.,
Joloba M.,
Koivula T.
Publication year - 2009
Publication title -
veterinary record
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.261
H-Index - 99
eISSN - 2042-7670
pISSN - 0042-4900
DOI - 10.1136/vr.164.21.655
Subject(s) - mycobacterium bovis , veterinary medicine , biology , microbiology and biotechnology , tuberculosis , mycobacterium tuberculosis , medicine , pathology
During a period of eight months, the carcases of 16,800 slaughter cattle were inspected at a city abattoir in Uganda. Eighty‐seven of them had tuberculosis‐like lesions and tissue samples were cultured. Only 17 cultures yielded acid‐fast bacilli; 11 of them were confirmed as Mycobacterium bovis and six as non‐tuberculous mycobacteria (NTM). GenoType Mycobacterium assays on the six NTM identified two as Mycobacterium fortuitum and one as Mycobacterium intracellulare , but three were unidentified. Characterisation of the M bovis isolates by spoligotyping and IS 6110 restriction fragment length polymorphism (RFLP) revealed that five of the six spoligotype patterns observed in the 11 strains had not been previously reported, and seven of the nine isolates typed by RFLP had multicopy number IS 6110 patterns. Six of the 11 infected carcases had multiple sites of infection, but none was condemned as unfit for human consumption.