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Recombinant antigen‐based dipstick elisa for the diagnosis of leptospirosis in dogs
Author(s) -
Dey S.,
Madhan Mohan C.,
Ramadass P.,
Nachimuthu K.
Publication year - 2007
Publication title -
veterinary record
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.261
H-Index - 99
eISSN - 2042-7670
pISSN - 0042-4900
DOI - 10.1136/vr.160.6.186
Subject(s) - dipstick , direct agglutination test , recombinant dna , antigen , antibody , leptospirosis , medicine , virology , agglutination (biology) , immunology , biology , serology , urine , biochemistry , gene
A recombinant LipL32 antigen‐based dipstick elisa was developed as a screening test for the detection of leptospiral antibodies in serum samples from dogs. The antibodies were detected by a change in the colour of the substrate solution when the recombinant antigen‐coated dipsticks were dipped into it. The relative sensitivity, specificity and accuracy of the test, compared with the standard microscopic agglutination test, were 95·9 per cent, 93·8 per cent and 94·8 per cent, respectively.