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M gene analysis of atypical strains of feline and canine coronavirus circulating in an Austrian animal shelter
Author(s) -
Benetka V.,
Kolodziejek J.,
Walk K.,
Rennhofer M.,
Möstl K.
Publication year - 2006
Publication title -
veterinary record
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.261
H-Index - 99
eISSN - 2042-7670
pISSN - 0042-4900
DOI - 10.1136/vr.159.6.170
Subject(s) - virology , genbank , coronavirus , phylogenetic tree , biology , feline infectious peritonitis , gene , cats , sequence analysis , genetics , covid-19 , medicine , disease , pathology , infectious disease (medical specialty)
Coronavirus‐positive samples of faeces collected in an Austrian animal shelter from 12 cats and 10 dogs were analysed by reverse transcriptase‐ pcr with primers amplifying a segment of the M protein gene, and by sequence analysis. In addition, the samples were subjected to S gene typing, using primers that differentiated between feline coronavirus ( fc o v ) types I and II. A phylogenetic analysis of the M gene sequences revealed not only clearly segregating canine coronavirus ( cc o v ) in the dogs, typical cc o v sequences and the recently described fc o v ‐like cc o v , but also at least two genetic clusters of fc o v in the cats, one species‐specific, the other more closely related to fc o v ‐like cc o v . The M gene sequences of these new feline strains had at most 88 per cent identity with the fc o v ‐like cc o v strain 259/01 and only up to 85 per cent with any fc o v sequence available in GenBank. In the phylogenetic tree they occupy an intermediate position between feline and canine coronaviruses.