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Canine adenovirus type 2 infection in four puppies with neurological signs
Author(s) -
Benetka V.,
Weissenböck H.,
Kudielka I.,
Pallan C.,
Rothmüller G.,
Möstl K.
Publication year - 2006
Publication title -
veterinary record
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.261
H-Index - 99
eISSN - 2042-7670
pISSN - 0042-4900
DOI - 10.1136/vr.158.3.91
Subject(s) - canine distemper , lung , bordetella bronchiseptica , spleen , virology , biology , respiratory system , nucleic acid , pathology , virus , pneumonia , medicine , immunology , anatomy , bacteria , biochemistry , genetics
Four nine‐ to 11‐week‐old puppies developed respiratory and neurological signs due to an infection with canine adenovirus type 2 ( CAV‐2 ); three of these were euthanased. They had moderate, diffuse pneumonia but there were no histological abnormalities in the central nervous system. Adenovirus‐specific nucleic acid was detected by PCR in samples of lung and brain and the amplified product was 99·8 per cent homologous with the CAV‐2 reference strain Toronto A26/61 . The positive PCR result was confirmed by in situ hybridisation in samples of lung, liver and spleen, but not in brain, and CAV was isolated in cell culture from lung material; PCR s for canine distemper virus and canine herpesvirus‐specific nucleic acids were negative, but large amounts of Bordetella bronchiseptica were isolated from lung material.