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Detection of lymph node micrometastases in malignant mammary tumours in dogs by cytokeratin immunostaining
Author(s) -
Matos A. J. F.,
Faustino A. M. R.,
Lopes C.,
Rutteman G. R.,
Gärtner F.
Publication year - 2006
Publication title -
veterinary record
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.261
H-Index - 99
eISSN - 2042-7670
pISSN - 0042-4900
DOI - 10.1136/vr.158.18.626
Subject(s) - haematoxylin , cytokeratin , pathology , immunostaining , eosin , immunohistochemistry , lymph node , medicine , stage (stratigraphy) , mammary gland , lymph , staining , breast cancer , micrometastasis , cancer , biology , paleontology
A series of 131 local and regional lymph nodes from 40 dogs with malignant mammary tumours were evaluated by staining with haematoxylin and eosin and immunohistochemically for antibodies to pancytokeratin ( AE1/AE3 ) and cytokeratin 14. The immunohistochemical tests detected occult micrometastases in 9·2 per cent of the lymph nodes that were negative by haematoxylin and eosin staining. Under the modified TNM classification of canine mammary tumours, these results raised the clinical stage of 12·5 per cent of the affected dogs. However, if the latest TNM classification of human breast cancer had been applied, none of the animals would have been reclassified.