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Detection of toxoplasmosis in experimentally infected goats by PCR
Author(s) -
Sreekumar C.,
Rao J. R.,
Mishra A. K.,
Ray D.,
Joshi P.,
Singh R. K.
Publication year - 2004
Publication title -
veterinary record
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.261
H-Index - 99
eISSN - 2042-7670
pISSN - 0042-4900
DOI - 10.1136/vr.154.20.632
Subject(s) - toxoplasmosis , lymph node , biology , lung , toxoplasma gondii , lymph , polymerase chain reaction , real time polymerase chain reaction , pathology , fetus , dna extraction , medicine , immunology , antibody , gene , pregnancy , biochemistry , genetics
PCR was used to diagnose toxoplasmosis in two pairs of Barbari goats infected by oral administration of doses of either 10 4 or 10 5 oocysts of Toxoplasma gondii . Blood and lymph node aspirates were collected from the infected goats and control goat at intervals, and tissues were also collected from a fetus that was aborted and a doe that died during the trial. Both processed and unprocessed samples were used for the PCR, using primers directed to the multicopy B1 gene. None of the blood samples was positive, but a specific signal was obtained from the lymph node aspirates after partial DNA extraction. Direct PCR of the lung, muscle and mesenteric lymph node of the doe and lung tissue of the aborted fetus yielded the target fragment. The simplified PCR protocols, including partial DNA extraction and direct assay of lung tissue, were effective for the diagnosis of toxoplasmosis.