Isolation of a glycoprotein E‐deleted bovine herpesvirus type 1 strain in the field | Zendy

Dispas M. | Zendy; Schynts F. | Zendy; Lemaire M. | Zendy; Letellier C. | Zendy; Vanopdenbosch E. | Zendy; Thiry E. | Zendy; Kerkhofs P. | Zendy

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Isolation of a glycoprotein E‐deleted bovine herpesvirus type 1 strain in the field

Author(s) -

Dispas M.,

Schynts F.,

Lemaire M.,

Letellier C.,

Vanopdenbosch E.,

Thiry E.,

Kerkhofs P.

Publication year - 2003

Publication title -

veterinary record

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.261

H-Index - 99

eISSN - 2042-7670

pISSN - 0042-4900

DOI - 10.1136/vr.153.7.209

Subject(s) - bovine herpesvirus 1 , virology , strain (injury) , nasal administration , vaccination , isolation (microbiology) , immunofluorescence , biology , virus , indirect immunofluorescence , glycoprotein , microbiology and biotechnology , herpesviridae , antibody , viral disease , immunology , anatomy

During a field trial to evaluate the efficacy of repeated vaccinations with bovine herpesvirus type 1 (BHV‐1) marker vaccines, a glycoprotein E (gE)‐negative BHV‐1 strain was isolated from the nasal secretions of two cows, eight months after vaccination with a gE‐negative live‐attenuated vaccine, initially given intranasally, then intramuscularly. The strain isolated was characterised using immunofluorescence, restriction analysis and CR. All the techniques used identified the isolated virus as a gE‐negative BHV‐1 phenotypically and genotypically identical to the Za strain used as a control.

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