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Isolation of a glycoprotein E‐deleted bovine herpesvirus type 1 strain in the field
Author(s) -
Dispas M.,
Schynts F.,
Lemaire M.,
Letellier C.,
Vanopdenbosch E.,
Thiry E.,
Kerkhofs P.
Publication year - 2003
Publication title -
veterinary record
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.261
H-Index - 99
eISSN - 2042-7670
pISSN - 0042-4900
DOI - 10.1136/vr.153.7.209
Subject(s) - bovine herpesvirus 1 , virology , strain (injury) , nasal administration , vaccination , isolation (microbiology) , immunofluorescence , biology , virus , indirect immunofluorescence , glycoprotein , microbiology and biotechnology , herpesviridae , antibody , viral disease , immunology , anatomy
During a field trial to evaluate the efficacy of repeated vaccinations with bovine herpesvirus type 1 (BHV‐1) marker vaccines, a glycoprotein E (gE)‐negative BHV‐1 strain was isolated from the nasal secretions of two cows, eight months after vaccination with a gE‐negative live‐attenuated vaccine, initially given intranasally, then intramuscularly. The strain isolated was characterised using immunofluorescence, restriction analysis and CR. All the techniques used identified the isolated virus as a gE‐negative BHV‐1 phenotypically and genotypically identical to the Za strain used as a control.