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Toxin types of Clostridium perfringens isolated from free‐ranging, semidomesticated reindeer in Norway
Author(s) -
Aschfalk A.,
ValentinWeigand P.,
Goethe R.,
Müller W.
Publication year - 2002
Publication title -
veterinary record
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.261
H-Index - 99
eISSN - 2042-7670
pISSN - 0042-4900
DOI - 10.1136/vr.151.7.210
Subject(s) - clostridium perfringens , toxin , enterotoxin , biology , microbiology and biotechnology , flock , feces , gene , virology , bacteria , escherichia coli , genetics , ecology
Samples of faeces were taken from 166 healthy domesticated reindeer ( Rangifer tarandus tarandus ) from three flocks in different reindeer husbandry districts in northern Norway and examined bacteriologically for the presence of Clostridium perfringens . The organism was isolated from 98 (59 per cent) of the reindeer. The isolates were classified into C perfringens toxin types by PKR analysis specific for the genes encoding the four major toxins (α, β, ϵ and ι) and were subclassified by the detection of the genes encoding C perfringens β2‐toxin and enterotoxin. All the isolates belonged to C perfringens toxin type A. In addition, 15 of the 98 isolates were PCR‐positive for the β2‐toxin gene, and two of the isolates had the the gene encoding for enterotoxin.