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Identification of Escherichia coli 01 57:H7‐ strains from pigs with postweaning diarrhoea and amplification of their virulence marker genes by PCR
Author(s) -
Osek J.
Publication year - 2002
Publication title -
veterinary record
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.261
H-Index - 99
eISSN - 2042-7670
pISSN - 0042-4900
DOI - 10.1136/vr.150.22.689
Subject(s) - intimin , escherichia coli , biology , microbiology and biotechnology , virulence , shiga toxin , gene , toxin , polymerase chain reaction , virology , enterobacteriaceae , genetics
Escherichia coli isolated from pigs with postweaning diarrhoea were examined by PCR for the presence of the 0157 rfb gene responsible for the biosynthesis of E coli 0157 lipopolysaccharide. Among the 372 isolates tested, 38 (10.2 per cent) were of the 0157 serogroup, but none of these possessed the H7 determinant. Further analysis of the E coli 0157 isolates revealed that seven of them had the genes responsible for the production of Shiga toxin 1 and eaeA intimin, four other strains had genes responsible for the production of Shiga toxin 2, and four other strains were positive for the enterohaemolysin gene.