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Identification of bluetongue virus serotype 2 (Corsican strain) by reverse‐transcriptase PCR reaction analysis of segment 2 of the genome
Author(s) -
Zientara S.,
Sailleau C.,
Dauphin G.,
Roquier C.,
Rémond E. M.,
Lebreton F.,
Hammoumi S.,
Dubois E.,
Agier C.,
Merle G.,
Bréard E.
Publication year - 2002
Publication title -
veterinary record
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.261
H-Index - 99
eISSN - 2042-7670
pISSN - 0042-4900
DOI - 10.1136/vr.150.19.598
Subject(s) - corsican , serotype , virology , genome , biology , virus , phylogenetic tree , strain (injury) , orbivirus , foot and mouth disease virus , identification (biology) , reverse transcriptase , polymerase chain reaction , reoviridae , genetics , gene , botany , philosophy , linguistics , anatomy , rotavirus
In October 2000, bluetongue virus was detected on the French island of Corsica. The disease was also reported in Sardinia, Calabria, Sicily and on the Spanish islands of Majorca and Minorca. This paper describes the use of molecular techniques for a rapid identification and serotype determination of serotype 2 of the virus. The nucleotide sequences of segments 2 and 7 of the genome of the Corsican strain were determined and its phylogenetic relationships are described.