Premium
Variations in cartilage catabolism in different equine joints in response to interleukin‐1 in vitro
Author(s) -
Fuller C. J.,
Barr A. R. S.,
Dieppe P. A.
Publication year - 2001
Publication title -
veterinary record
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.261
H-Index - 99
eISSN - 2042-7670
pISSN - 0042-4900
DOI - 10.1136/vr.148.7.204
Subject(s) - cartilage , interphalangeal joint , explant culture , glycosaminoglycan , medicine , metacarpus , metacarpophalangeal joint , anatomy , in vitro , chemistry , thumb , biochemistry
An explant system was used to investigate the hypothesis that cartilage from different equine joints might respond differently to challenge with interleukin‐1 α (L‐1 α). Pairs of normal cartilage samples were taken from the metacarpophalangeal, proximal interphalangeal and distal interphalangeal joints of six horses. One of each pair was stimulated with 10 ng/ml human recombinant IL‐1 α for three days, and the supernatants and remaining cartilage explants were analysed for their total content of glycosaminoglycans. A significantly higher percentage of glycosaminoglycans was released from the cartilage of the proximal and distal interphalangeal joints than from the metacarpophalangeal joint.