Premium
First confirmed canine case of Ehrlichia canis infection in Japan
Author(s) -
Suto Y.,
Suto A.,
Inokuma H.,
Obayashi H.,
Hayashi T.
Publication year - 2001
Publication title -
veterinary record
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.261
H-Index - 99
eISSN - 2042-7670
pISSN - 0042-4900
DOI - 10.1136/vr.148.26.809
Subject(s) - ehrlichia canis , canis , serology , spleen , polymerase chain reaction , ehrlichia , biology , virology , ehrlichiosis , 16s ribosomal rna , pathology , antibody , gene , immunology , medicine , genetics , paleontology , tick
An 11‐year‐old castrated Pekinese dog that had been moved from Indonesia to Japan eight years previously was diagnosed with an Ehrlichia canis infection by haematological characteristics (normocytic anaemia, mild thrombocytopenia and hypergammaglobulinaemia) and serological findings (antibody titre to E canis 1:3200 or more). The dog did not respond to treatment with tetracycline and died from renal failure. The diagnosis was confirmed postmortem by pathological evaluation and polymerase chain reaction (PcR) followed by sequencing of the 16S rRNA gene. Typical morulae of Ehrlichia were detected in the cytoplasm of macrophages in spleen tissue by immunohistological staining. Ehrlichia ‐like organisms were also detected in the spleen by electron microscopy. E canis ‐specific PCR analysis of DNA extracted from the spleen gave a positive signal, and sequence analysis of the fragment revealed that it was identical to part of the 16S rRNA gene of E canis . The dog was the first confirmed clinical case of E canis infection in Japan.