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Epidemiological and diagnostic observations following the elimination of porcine reproductive and respiratory syndrome virus from a breeding herd of pigs by the test and removal protocol
Author(s) -
Dee S. A.,
Molitor T. W.,
Rossow K. D.
Publication year - 2000
Publication title -
veterinary record
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.261
H-Index - 99
eISSN - 2042-7670
pISSN - 0042-4900
DOI - 10.1136/vr.146.8.211
Subject(s) - herd , porcine reproductive and respiratory syndrome virus , virus , biology , veterinary medicine , virology , respiratory system , polymerase chain reaction , arterivirus , medicine , zoology , covid-19 , gene , anatomy , biochemistry , disease , infectious disease (medical specialty)
The protocol of test and removal for the elimination of porcine reproductive and respiratory syndrome (PRRS) virus was applied to an 825‐sow breeding herd. All the adult animals were tested and serum samples analysed by ELISA and polymerase chain reaction (PCR). Eighty‐eight animals (10.7 per cent) were removed from the herd and, of these, three were ELISA‐positive and PcR‐positive, and 85 were ELISA‐positive and PCRnegative. They tended to be either individual sows, or groups of four to six animals housed in adjacent gestation stalls. Four of the ELISA‐positive, PCR‐negative sows were slaughtered and PRRS virus nucleic acid was detected in a sample of sternal lymph node from one of them. After the completion of the test and removal protocol, the breeding and finishing populations were monitored for 12 consecutive months by ELISA. The 960 samples taken were negative for PRRS virus antibodies.