Premium
Detection of DNA restriction fragment polymorphisms in Streptococcus equi
Author(s) -
Takai S.,
Yashiro H.,
Ishii C.,
Tsubaki S.,
Anzai T.,
Wada R.,
Timoney J. F.
Publication year - 2000
Publication title -
veterinary record
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.261
H-Index - 99
eISSN - 2042-7670
pISSN - 0042-4900
DOI - 10.1136/vr.146.6.159
Subject(s) - restriction enzyme , streptococcus equi , typing , biology , restriction fragment length polymorphism , outbreak , genotype , strain (injury) , pulsed field gel electrophoresis , restriction fragment , subspecies , microbiology and biotechnology , virology , dna , genetics , gene , bacteria , paleontology , anatomy
Large‐restriction‐fragment (LRF) polymorphisms in Streptococcus equi ( S equi subspecies equi ) were studied by pulsed‐field gel electrophoresis. Five or six chromosomal fragments of between 194 and 915 kb were separated by digestion with the restriction endonuclease Nof l. All 20 isolates of S equi , including 12 from independent Japanese outbreaks, four from independent American outbreaks, two from a single Irish outbreak, us vaccine strain F43, and type strain NCTC 9682 were successfully typed. Seven distinctive, reproducible and stable types were identified. The 12 Japanese isolates collected between 1992 and 1998 were of LRF type 11 suggesting that they were derived from the same source. The remaining eight isolates were of six types. The results indicate that LRF typing should be a useful technique for investigating the source and transmission of S equi .