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Comparison of in vitro activity of danofloxacin, florfenicol, oxytetracycline, spectinomycin and tilmicosin against recent field isolates of Mycoplasma bovis
Author(s) -
Ayling R. D.,
Baker S. E.,
Nicholas R. A. J.,
Peek M. L.,
Simon A. J.
Publication year - 2000
Publication title -
veterinary record
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.261
H-Index - 99
eISSN - 2042-7670
pISSN - 0042-4900
DOI - 10.1136/vr.146.26.745
Subject(s) - danofloxacin , spectinomycin , florfenicol , oxytetracycline , tilmicosin , tylosin , minimum inhibitory concentration , microbiology and biotechnology , biology , veterinary medicine , antibiotics , medicine , antibiotic resistance , enrofloxacin , ciprofloxacin
The minimum inhibitory concentrations (MICs) and minimum mycoplasmacidal concentrations (MMCs) of danofloxacin, florfenicol, oxytetracycline, spectinomycin and tilmicosin against 62 recent British field isolates of Mycoplasma bovis were determined in vitro by a broth microdilution method. The isolates were most susceptible to danofloxacin with MIC 90 and MMC 90 values of 0.5 μg/ml and 1.0 μg/ml, respectively. They were less susceptible to florfenicol with a MIC 90 of 16 μg/ml and MMC 90 of 32 μg/ml. Oxytetracycline and spectinomycin had only a limited effect against the majority of isolates tested with MIC 50 s of 32 μg/ml and 4 μg/ml, respectively and MIC 90 s of 64 μg/ml and more than 128 μg/ml, respectively. Nearly 20 per cent of the isolates were highly resistant to spectinomycin, and tilmicosin was ineffective, with 92 per cent of the isolates having MIC values of 128 μg/ml or greater. There was no evidence of resistance by M bovis to danofloxacin.