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Application of the Veftest 8008 system for the biochemical analysis of vervet monkey plasma
Author(s) -
Ngure R. M.,
Gateri L. M.,
Ngotho J. M.,
Ndung'u J. M.
Publication year - 2000
Publication title -
veterinary record
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.261
H-Index - 99
eISSN - 2042-7670
pISSN - 0042-4900
DOI - 10.1136/vr.146.21.612
Subject(s) - lactate dehydrogenase , creatine kinase , alkaline phosphatase , albumin , creatinine , blood urea nitrogen , alanine aminotransferase , urea , globulin , blood proteins , medicine , haemolysis , creatine , alanine transaminase , endocrinology , aspartate transaminase , biology , biochemistry , enzyme , immunology
A clinical biochemistry analyser designed specifically for veterinary use was used to analyse plasma samples from 24 vervet monkeys (Cercopithecus aethiops). Two millilitres of heparinised blood was collected from each of the 24 monkeys on four occasions at intervals of one week. Plasma was separated and analysed for the concentrations of triglycerides, cholesterol, total proteins, albumin, globulins, creatinine and blood urea nitrogen (BUN) and the activities of alkaline phosphatase (AP), lactate dehydrogenase (LDH), alanine aminotrasferase (ALT), aspartate aminotransferase (AST), and creatine kinase (CK). The tests were easy to perform, used small volumes of plasma, and yielded consistent results for most of the analytes. The activities of CK and AP, but not AST, appeared to be influenced by haemolysis, and there were significant individual variations in the activity of LDH.