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Evaluation of a commercial ELISA for detecting serum antibody to Neospora caninum in cattle
Author(s) -
Williams D. J. L.,
Davison H. C.,
Helmick B.,
McGarry J.,
Guy F.,
Otter A.,
Trees A. J.
Publication year - 1999
Publication title -
veterinary record
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.261
H-Index - 99
eISSN - 2042-7670
pISSN - 0042-4900
DOI - 10.1136/vr.145.20.571
Subject(s) - neospora caninum , neospora , antibody , herd , biology , virology , direct fluorescent antibody , veterinary medicine , medicine , immunology , toxoplasma gondii
A commercially available serum antibody detection enzyme‐linked immunosorbent assay for Neospora caninum in cattle was evaluated against an immunofluorescent antibody test (IFAT) by applying it to 397 sera from normal adult cattle, 352 sera from cattle which had recently aborted, and 422 sera from two herds which had a history of N caninum ‐associated abortions. It was evaluated in two laboratories and showed high reproducibility, repeatability and almost perfect or substantial agreement with the IFAT.