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Serodiagnosis of goat warble fly infestation by Przhevalskiana silenus with a commercial ELISA kit
Author(s) -
Otranto D.,
Boulard C.,
Giangaspero A.,
Caringella M. P.,
Rimmele D.,
Puccini V.
Publication year - 1999
Publication title -
veterinary record
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.261
H-Index - 99
eISSN - 2042-7670
pISSN - 0042-4900
DOI - 10.1136/vr.144.26.726
Subject(s) - infestation , veterinary medicine , biology , virology , medicine , agronomy
Cross‐reactivity between Hypoderma lineatum antigen and anti‐ Przhevalskiana silenus antibodies has been demonstrated by an ELISA technique. To evaluate the applicability of a commercial ELISA kit for the immunodiagnosis of goat warble fly infestation, different dilutions of serum and conjugate were tested, the development of antibody to P silenus in naturally infested goats was studied, and the results were compared with an ELISA technique using an antigen extracted from the first instar larvae of H lineatum . The best results were obtained with a serum dilution of 1:50; with both techniques the highest antibody concentration were recorded in October, November and December. In view of the confirmed cross‐reactivity between H lineatum antigen and anti‐ P silenus antibodies, and the simplicity and rapidity of the assay, the commercial ELISA kit can be considered as a useful tool for the diagnosis of goat warble fly infestation.