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Diagnosis of canine leishmaniasis by a polymerase chain reaction technique
Author(s) -
Roura X.,
Sánchez A.,
Ferrer L.
Publication year - 1999
Publication title -
veterinary record
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.261
H-Index - 99
eISSN - 2042-7670
pISSN - 0042-4900
DOI - 10.1136/vr.144.10.262
Subject(s) - kinetoplast , meglumine antimoniate , polymerase chain reaction , leishmaniasis , leishmania , allopurinol , canine leishmaniasis , medicine , leishmania infantum , cutaneous leishmaniasis , virology , visceral leishmaniasis , biology , dna , immunology , pathology , parasite hosting , genetics , gene , world wide web , computer science
A polymerase chain reaction (PCR) technique for the diagnosis of canine leishmaniasis on bone marrow samples was developed which amplified a 120 bp DNA fragment of the Leishmania kinetoplast DNA, common to all Leishmania species. Forty‐five of 46 dogs in which leishmaniasis had been diagnosed were positive with the PCR technique, whereas none of 41 healthy dogs gave a positive result. Fifteen dogs with leishmaniasis that had been treated for six months with N‐methylglucamine antimoniate and allopurinol were also investigated. Seven were positive, implying that they remained infected despite the resolution of their clinical signs.