Comparison of polyclonal, monoclonal and protein G peroxidase conjugates in an enzyme‐linked immunosorbent assay for the diagnosis of  Brucella ovis  in sheep | Zendy

Marín C. M. | Zendy; AlonsoUrmeneta B. | Zendy; Moriyón I. | Zendy; PérezGómez S. | Zendy; Blasco J. M. | Zendy

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Comparison of polyclonal, monoclonal and protein G peroxidase conjugates in an enzyme‐linked immunosorbent assay for the diagnosis of Brucella ovis in sheep

Author(s) -

Marín C. M.,

AlonsoUrmeneta B.,

Moriyón I.,

PérezGómez S.,

Blasco J. M.

Publication year - 1998

Publication title -

veterinary record

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.261

H-Index - 99

eISSN - 2042-7670

pISSN - 0042-4900

DOI - 10.1136/vr.143.14.390

Subject(s) - brucella melitensis , polyclonal antibodies , brucella , monoclonal antibody , conjugate , ovis , monoclonal , peroxidase , microbiology and biotechnology , biology , brucellosis , enzyme , virology , chemistry , antigen , antibody , immunology , biochemistry , mathematical analysis , ecology , mathematics

The sensitivities of three commercial peroxidase conjugates (polyclonal anti‐sheep IgG, recombinant protein G and a monoclonal anti‐ruminant IgG 1 ) in an enzyme‐linked immunosorbent assay for Brucella ovis were evaluated. The monoclonal and protein G conjugates reduced, but did not totally remove, the characteristic background reactivity observed with the sera from brucella‐free sheep. The protein G conjugate provided the best sensitivity, similar to that obtained with the classical gel diffusion test. Both tests were highly specific when testing sera from brucella‐free animals but detected as positive a large proportion of sera from both Brucella melitensis ‐infected and B melitensis Rev 1‐vaccinated sheep.

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