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Comparison of polyclonal, monoclonal and protein G peroxidase conjugates in an enzyme‐linked immunosorbent assay for the diagnosis of Brucella ovis in sheep
Author(s) -
Marín C. M.,
AlonsoUrmeneta B.,
Moriyón I.,
PérezGómez S.,
Blasco J. M.
Publication year - 1998
Publication title -
veterinary record
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.261
H-Index - 99
eISSN - 2042-7670
pISSN - 0042-4900
DOI - 10.1136/vr.143.14.390
Subject(s) - brucella melitensis , polyclonal antibodies , brucella , monoclonal antibody , conjugate , ovis , monoclonal , peroxidase , microbiology and biotechnology , biology , brucellosis , enzyme , virology , chemistry , antigen , antibody , immunology , biochemistry , mathematical analysis , ecology , mathematics
The sensitivities of three commercial peroxidase conjugates (polyclonal anti‐sheep IgG, recombinant protein G and a monoclonal anti‐ruminant IgG 1 ) in an enzyme‐linked immunosorbent assay for Brucella ovis were evaluated. The monoclonal and protein G conjugates reduced, but did not totally remove, the characteristic background reactivity observed with the sera from brucella‐free sheep. The protein G conjugate provided the best sensitivity, similar to that obtained with the classical gel diffusion test. Both tests were highly specific when testing sera from brucella‐free animals but detected as positive a large proportion of sera from both Brucella melitensis ‐infected and B melitensis Rev 1‐vaccinated sheep.