Premium
Detection of Newcastle disease virus‐specific antibodies in ostrich sera by three serological methods
Author(s) -
Koch G.,
Czifra G.,
Engström B. E.
Publication year - 1998
Publication title -
veterinary record
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.261
H-Index - 99
eISSN - 2042-7670
pISSN - 0042-4900
DOI - 10.1136/vr.143.1.10
Subject(s) - virology , newcastle disease , serology , antibody , virus , neutralization , serotype , haemagglutination inhibition , hemagglutination , biology , monoclonal antibody , immunology
Sera from 211 ostriches were tested for the presence of Newcastle disease virus (NDv)‐specific antibodies by the virus neutralisation test, the haemagglutination inhibition (HI) test and a recently developed avian paramyxovirus serotype 1 (PMv‐1) specific monoclonal antibody blocking ELISA (b‐ELISA). The virus neutralisation test was used as the reference for the estimation of the sensitivity and specificity of the b‐ELISA and HI tests. Of the 211 sera, 140 contained NDv‐specific neutralising antibodies, 130 were positive by the HI test and 122 by the b‐ELISA. The sensitivity, specificity and predictive accuracy of the HI and b‐ELISA tests relative to the virus neutralisation test were similar. The good agreement between the HI and b‐ELISA test (κ = 0.85) suggested that the two methods are interchangeable.