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Development of a slide ELISA for canine leishmaniasis and comparison with four serological tests
Author(s) -
Vercammen F.,
Berkvens D.,
Le Ray D.,
Jacquet D.,
Vervoort T.
Publication year - 1997
Publication title -
veterinary record
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.261
H-Index - 99
eISSN - 2042-7670
pISSN - 0042-4900
DOI - 10.1136/vr.141.13.328
Subject(s) - serology , canine leishmaniasis , virology , leishmaniasis , biology , medicine , antibody , immunology , visceral leishmaniasis , leishmania infantum
A slide ELISA for canine leishmaniasis was developed by using promastigotes of Leishmania infantum , and compared with microimmunodiffusion, immunoelectrophoresis, direct agglutination and indirect immunofluorescence assays. The sensitivity of all the tests was 100 per cent. The specificity of the direct agglutination test was 95 per cent but it was 100 per cent for the three other tests. There was also a positive correlation and a high level of concordance between the titres measured by the different tests.