Partial identification of spirochaetes from two dairy cows with digital dermatitis by polymerase chain reaction analysis of the 16S ribosomal RNA gene | Zendy

Rijpkema S. G. T. | Zendy; David G. P. | Zendy; Hughes S. L. | Zendy; Woodward M. J. | Zendy

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Partial identification of spirochaetes from two dairy cows with digital dermatitis by polymerase chain reaction analysis of the 16S ribosomal RNA gene

Author(s) -

Rijpkema S. G. T.,

David G. P.,

Hughes S. L.,

Woodward M. J.

Publication year - 1997

Publication title -

veterinary record

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.261

H-Index - 99

eISSN - 2042-7670

pISSN - 0042-4900

DOI - 10.1136/vr.140.10.257

Subject(s) - 16s ribosomal rna , spirochaete , polymerase chain reaction , ribosomal rna , identification (biology) , gene , biology , digital polymerase chain reaction , microbiology and biotechnology , genetics , bacteria , botany

Specimens taken postmortem from typical lesions of digital dermatitis in two dairy cows were tested by the polymerase chain reaction (PCR) for the presence of a spirochaetal 16S rRNA gene. Seven different assays detected the gene in the samples from both cows. Two of the PCR products were sequenced and a comparison of the nucleotide sequences revealed that the spirochaete belonged to the genus Treponema and was closely related to Treponema denticola . A PCR specific for the detection of the digital dermatitis‐associated treponeme was developed.

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