Premium
Partial identification of spirochaetes from two dairy cows with digital dermatitis by polymerase chain reaction analysis of the 16S ribosomal RNA gene
Author(s) -
Rijpkema S. G. T.,
David G. P.,
Hughes S. L.,
Woodward M. J.
Publication year - 1997
Publication title -
veterinary record
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.261
H-Index - 99
eISSN - 2042-7670
pISSN - 0042-4900
DOI - 10.1136/vr.140.10.257
Subject(s) - 16s ribosomal rna , spirochaete , polymerase chain reaction , ribosomal rna , identification (biology) , gene , biology , digital polymerase chain reaction , microbiology and biotechnology , genetics , bacteria , botany
Specimens taken postmortem from typical lesions of digital dermatitis in two dairy cows were tested by the polymerase chain reaction (PCR) for the presence of a spirochaetal 16S rRNA gene. Seven different assays detected the gene in the samples from both cows. Two of the PCR products were sequenced and a comparison of the nucleotide sequences revealed that the spirochaete belonged to the genus Treponema and was closely related to Treponema denticola . A PCR specific for the detection of the digital dermatitis‐associated treponeme was developed.