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Detection of Salmonella enteritidis in eggs by the polymerase chain reaction
Author(s) -
Woodward M. J.,
Kirwan S. E. S.
Publication year - 1996
Publication title -
veterinary record
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.261
H-Index - 99
eISSN - 2042-7670
pISSN - 0042-4900
DOI - 10.1136/vr.138.17.411
Subject(s) - salmonella enteritidis , polymerase chain reaction , salmonella , microbiology and biotechnology , biology , real time polymerase chain reaction , gene , chemistry , bacteria , biochemistry , genetics
A polymerase chain reaction (PCR) for the specific detection of the gene sequence, sefA, encoded by all isolates of Salmonella enteritidis , was developed. The PCR could detect as few as four S enteritidis washed bacterial cells but egg contents inhibited the PCR. Eggs spiked with 50 S enteritidis bacterial cells were homogenised, inoculated into buffered peptone water and grown at 37°C for 16 hours, when the PCR was successful. A positive internal control was developed to differentiate between true and false negative PCR results for the detection of S enteritidis . In a limited trial of the egg handling procedures and the PCR, one of 250 chickens' eggs from retail outlets was found to be contaminated with S enteritidis .