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Use of polymerase chain reaction in the diagnosis of tuberculosis in cats and dogs
Author(s) -
Aranaz A.,
Liébana E.,
Pickering X.,
Novoa C.,
Mateos A.,
Domínguez L.
Publication year - 1996
Publication title -
veterinary record
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.261
H-Index - 99
eISSN - 2042-7670
pISSN - 0042-4900
DOI - 10.1136/vr.138.12.276
Subject(s) - polymerase chain reaction , cats , tuberculosis , histopathology , mycobacterium tuberculosis , dna extraction , biology , real time polymerase chain reaction , mycobacterium tuberculosis complex , microbiological culture , microbiology and biotechnology , pathology , medicine , bacteria , gene , genetics
Samples from four dogs and four cats suspected of having tuberculosis were processed for histopathology, bacterial culture and polymerase chain reaction (PCR). A simple, rapid method for the extraction of DNA from tissue samples was used in two PCR assays designed to confirm the diagnosis of tuberculosis. The PCR assays detected all the culture‐positive samples from these animals and no false positive results were obtained. The PCR technique was successful for the direct detection of organisms from the Mycobacterium tuberculosis complex and reduced the time needed for a diagnosis to two days.