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Surveying bovine digital dermatitis and non‐healing bovine foot lesions for the presence of Fusobacterium necrophorum, Porphyromonas endodontalis and Treponema pallidum
Author(s) -
Staton Gareth James,
Sullivan Leigh Emma,
Blowey Roger W,
Carter Stuart D,
Evans Nicholas James
Publication year - 2020
Publication title -
veterinary record
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.261
H-Index - 99
eISSN - 2042-7670
pISSN - 0042-4900
DOI - 10.1136/vr.105628
Subject(s) - treponema , fusobacterium necrophorum , lesion , microbiology and biotechnology , biology , lameness , pathology , hoof , medicine , syphilis , immunology , surgery , anatomy , human immunodeficiency virus (hiv)
Background Non‐healing bovine foot lesions, including non‐healing white line disease, non‐healing sole ulcer and toe necrosis, are an increasingly important cause of chronic lameness that are poorly responsive to treatment. Recent studies have demonstrated a high‐level association between these non‐healing lesions and the Treponema phylogroups implicated in bovine digital dermatitis (BDD). However, a polymicrobial aetiology involving other gram‐stain‐negative anaerobes is suspected. Methods A PCR‐based bacteriological survey of uncomplicated BDD lesions (n=10) and non‐healing bovine foot lesions (n=10) targeting Fusobacterium necrophorum , Porphyromonas endodontalis, Dichelobacter nodosus and Treponema pallidum/T. paraluiscuniculi was performed. Results P. endodontalis DNA was detected in 80.0% of the non‐healing lesion biopsies (p=<0.001) but was entirely absent from uncomplicated BDD lesion biopsies. When compared to the BDD lesions, F. necrophorum was detected at a higher frequency in the non‐healing lesions (33.3% vs 70.0%, respectively), whereas D. nodosus was detected at a lower frequency (55.5% vs 20.0%, respectively). Conversely, T. pallidum/T. paraluiscuniculi DNA was not detected in either lesion type. Conclusion The data from this pilot study suggest that P. endodontalis and F. necrophorum should be further investigated as potential aetiological agents of non‐healing bovine foot lesions. A failure to detect syphilis treponemes in either lesion type is reassuring given the potential public health implications such an infection would present.