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Analysis of bovine viral diarrhoea virus: Biobank and sequence database to support eradication in Scotland
Author(s) -
Russell G. C.,
Grant D. M.,
Lycett S.,
Bachofen C.,
Caldow G. L.,
Burr P. D.,
Davie K.,
Ambrose N.,
Gunn G. J.,
Zadoks R. N.
Publication year - 2017
Publication title -
veterinary record
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.261
H-Index - 99
eISSN - 2042-7670
pISSN - 0042-4900
DOI - 10.1136/vr.104072
Subject(s) - biobank , virology , sequence (biology) , livestock , biology , virus , sequence analysis , untranslated region , veterinary medicine , geography , genetics , medicine , gene , rna , ecology
Samples from bovine viral diarrhoea virus (BVDV)—positive cattle were gathered by Scottish diagnostic laboratories and used to produce a Biobank of samples with associated location and identification data in support of the Scottish BVDV eradication scheme. The samples were subject to direct amplification and sequencing of the 5′‐untranslated region (5′‐UTR) to define the viral types and subtypes present. From 2693 samples collected prior to 2016, approximately 2300 sequences were obtained, representing 8 BVDV type 1 subtypes. No BVDV type 2 samples were detected. The samples came from all regions of the UK but 66 per cent were from Scotland. Analysis of the sequences showed great diversity in the 5′‐UTR, with 1206 different sequences. Many samples carried virus with identical 5′‐UTR sequences; often from single locations, but there were also examples of the same sequence being obtained from samples at several different locations. This work provides a resource that can be used to analyse the movement of BVDV strains both within Scotland and between Scotland and other nations, particularly in the latter stages of the Scottish eradication programme, and so inform the advice available to both livestock keepers and policymakers.