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Development of rapid immunochromatographic strip test for the detection of porcine epidemic diarrhoea virus
Author(s) -
Lyoo KwangSoo,
Yeom Minjoo,
Kim Jungho,
Kim Donghyuk,
Ha Gunwoo,
Na Woonsung,
Le Van Phan,
Song Daesub
Publication year - 2017
Publication title -
veterinary record
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.261
H-Index - 99
eISSN - 2042-7670
pISSN - 0042-4900
DOI - 10.1136/vr.103959
Subject(s) - porcine epidemic diarrhea virus , virology , medicine , veterinary medicine , virus , biology
Porcine epidemic diarrhoea virus (PEDV) causes acute and severe watery diarrhoea and dehydration, as well as 50–100 per cent mortality in piglets. For the PEDV diagnosis, a rapid test kit that is specific and sensitive to PEDV is critical to monitor this disease at pig farms. The present study aimed to develop an immunochromatographic assay (ICA) strip test for detecting PEDV in faecal swabs. The newly developed diagnostic test showed a detection limit of 10 4.0 TCID 50 /ml of PEDV. Using faecal swab samples, the relative sensitivity and specificity of the ICA kit were 95.0 per cent and 98.6 per cent, respectively, compared with those of real‐time RT‐PCR. In samples from piglets experimentally infected with PEDV, the results showed 100 per cent agreement with those found by real‐time RT‐PCR. Our developed test strip will be useful for rapid diagnosis and can be used for epidemiological surveillance of PEDV infection.