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Interlaboratory ring trial to evaluate CFT proficiency of European laboratories for diagnosis of glanders in equids
Author(s) -
Laroucau K.,
Colaneri C.,
Jaÿ M.,
Corde Y.,
Drapeau A.,
Durand B.,
Zientara S.,
Beck C.
Publication year - 2016
Publication title -
veterinary record
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.261
H-Index - 99
eISSN - 2042-7670
pISSN - 0042-4900
DOI - 10.1136/vr.103617
Subject(s) - complement fixation test , medicine , antibody , virology , immunology , serology
To evaluate the routine complement fixation test (CFT) used to detect Burkholderia mallei antibodies in equine sera, an interlaboratory proficiency test was held with 24 European laboratories, including 22 National Reference Laboratories for glanders. The panels sent to participants were composed of sera with or without B mallei antibodies. This study confirmed the reliability of CFT and highlighted its intralaboratory reproducibility. However, the sensitivity of glanders serodiagnosis and laboratory proficiency may be improved by standardising critical reagents, including antigens, and by developing a standard B mallei serum.