Open AccessMolecular characterisation of Mycoplasma hyorhinis isolated from pigs using pulsed‐field gel electrophoresis and 16S rRNA sequencing
Author(s) -
Yamaguti Maurício,
Oliveira Rosângela C,
Marques Lucas M,
Buzinhani Melissa,
Buim Marcos R,
Neto Renata L,
Guimarães Ana Márcia S,
Timenetsky Jorge
Publication year - 2015
Publication title -
veterinary record open
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.504
H-Index - 12
ISSN - 2052-6113
DOI - 10.1136/vetreco-2014-000093
Subject(s) - biology , 16s ribosomal rna , pulsed field gel electrophoresis , microbiology and biotechnology , ribosomal rna , mycoplasma , genotype , mycoplasma hyopneumoniae , gene , genetics
Economic loss in pig breeding is common due to respiratory disorders, and Mycoplasma hyopneumoniae and Mycoplasma hyorhinis , namely, are the most common infectious agents. The aim of this study is to recover these mollicutes and detect their genotypic variations by pulsed‐field gel electrophoresis (PFGE) and sequencing the 16 s rRNA gene. One hundred and twenty‐six swabs from tonsil and nasal mucus of pigs with respiratory disorders were analysed. A total of 78 lungs were sampled, as well as two trachea and two tonsils obtained from animals with respiratory disorder. A total of 59 isolates were obtained: 1 (1.70 per cent) of M hyopneumoniae , 2 (3.40 per cent) of Mycoplasma flocculare and 56 (94.90 per cent) of M hyorhinis . The PFGE for M hyorhinis showed 10 profiles with enzyme Ava I and 9 profiles with Xho I. A low polymorphism of the 16sRNS gene was detected in M hyorhinis isolates compared with the type strain in the GenBank. M hyorhinis isolates of different herds showed a large heterogenicity with enzymes Ava I and Xho I. The sequencing of the 16S rRNA gene allowed for analysing the interspecific and intraspecific variations of isolated mycoplasmas.