Open AccessAntigenic and molecular characterisation of Border disease virus associated with high mortality in lambs in Spain
Author(s) -
Vega S.,
Rosell R.,
Orden J. A.,
Pérez T.,
Marín C.,
González S.,
Marco I.,
Cabezón O.,
Fuente R.
Publication year - 2015
Publication title -
veterinary record open
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.504
H-Index - 12
eISSN - 2052-6113
pISSN - 2399-2050
DOI - 10.1136/vetreco-2014-000048
Subject(s) - outbreak , epidemiology , virology , biology , molecular epidemiology , virus , disease , strain (injury) , genotype , genetics , gene , medicine , pathology , anatomy
Border disease virus (BDV) causes congenital disorders in sheep and results in severe, but underestimated, economic losses worldwide. However, information about BDV strains affecting several ruminants worldwide is scarce. Therefore, antigenic and genetic classification of isolates from different geographical regions is important to enhance the knowledge of the epidemiology of BDV. Materials and methods Five pestiviruses isolated from lambs in an epidemic outbreak with an unusually high mortality in Spain in 1997 were characterised antigenically with a panel of monoclonal antibodies and genetically by sequencing within the 50 untranslated (50UTR) region of the genome. Results All the isolates were classified as BDV and showed a high homology with the Aveyron strain (Av), which was associated with an epidemic reported in sheep from the Aveyron region of France in 1984. Conclusions Classification of the isolates from this study provides valuable information on the molecular epidemiology of BDV.