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Detection of Treponema pedis associated with digital dermatitis in Mediterranean buffalo ( Bubalus bubalis )
Author(s) -
Guccione Jacopo,
Della Valle Giovanni,
Carcasole Christian,
Kuhnert Peter,
Alsaaod Maher
Publication year - 2018
Publication title -
veterinary record case reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.165
H-Index - 4
ISSN - 2052-6121
DOI - 10.1136/vetreccr-2018-000674
Subject(s) - milking , medicine , veterinary medicine , bubalus , crossbreed , hoof , lameness , mastitis , zoology , pathology , surgery , biology , anatomy , ecology
Clinical outcomes compatible with digital dermatitis (DD) were observed in a second lactation, milking Mediterranean buffalo (MB). The animal was sharing the free stall with other 99 milking MBs separated by a fence from a pen harbouring 40 milking crossbreed dairy cows. The macroscopic lesion observed on the right rear limb was categorised as M4.1 stage and associated with a severe lameness (locomotion score 5). In the same farm, DD was also observed in 14/40 cows. Nested PCR analysis of genomic DNA isolated from the centre lesion’s biopsy showed the presence of Treponema pedis . Sequence analysis of 494 bp of the PCR fragment revealed 100 per cent sequence identity to the T pedis ‐type strain, confirming the PCR result’s specificity and for the first time the presence of the bacterium in MB. In order to establish the spread of the DD, a farm inspection was performed to identify risk factors and preventive strategies.