Open AccessHigh-resolution scanning electron microscopy for the analysis of three-dimensional ultrastructure of clots in acute ischemic stroke
Author(s) -
Oana Madalina Mereuta,
Seán Fitzgerald,
Trace Christensen,
Adam Jaspersen,
Daying Dai,
Mehdi Abbasi,
Tejaswini Puttappa,
R Kadirvel,
David F. Kallmes,
Karen Doyle,
Waleed Brinjikji
Publication year - 2020
Publication title -
journal of neurointerventional surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.652
H-Index - 55
eISSN - 1759-8486
pISSN - 1759-8478
DOI - 10.1136/neurintsurg-2020-016709
Subject(s) - fibrin , thrombus , ultrastructure , medicine , pathology , immunohistochemistry , scanning electron microscope , von willebrand factor , electron microscope , stroke (engine) , platelet , biomedical engineering , surgery , materials science , immunology , mechanical engineering , physics , optics , composite material , engineering
Characterization of acute ischemic stroke (AIS) clots has typically focused on two-dimensional histological analysis of the thrombus. The three-dimensional (3D) architecture and distribution of components within emboli have not been fully investigated. The aim of this study was to examine the composition and microstructure of AIS clots using histology and serial block-face scanning electron microscopy (SBFSEM).