In situ amplification of oestrogen receptor alpha mRNA in breast cancer cell lines and tumours | Zendy

Laurence Lespagnard | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

In situ amplification of oestrogen receptor alpha mRNA in breast cancer cell lines and tumours

Author(s) -

Laurence Lespagnard

Publication year - 2001

Publication title -

molecular pathology

Language(s) - English

Resource type - Journals

eISSN - 1472-4154

pISSN - 1366-8714

DOI - 10.1136/mp.54.3.197

Subject(s) - in situ , cell culture , messenger rna , breast cancer , in vivo , ex vivo , in vitro , cancer research , cell , in situ hybridization , microbiology and biotechnology , biology , reverse transcription polymerase chain reaction , pathology , chemistry , cancer , medicine , gene , biochemistry , genetics , organic chemistry

The aim of this work was to develop a direct in situ reverse transcription polymerase chain reaction (in situ RT-PCR) assay for the detection of oestrogen receptor alpha (ERalpha) mRNA on in vitro cell lines and breast tumour cell smears. ERalpha mRNA amplification was performed on MCF-7 (ERalpha positive) and MDA-MB-231 (ERalpha negative) cell lines as well as on 51 cytological smears of breast tumour samples from patients. The in situ amplification of mRNA in cell lines and ex vivo breast tumours was successful. However, finding an equilibrium between optimal cell morphology and PCR performance varied with each tumour, leading to difficulty in standardisation for daily practice. Nonetheless, in situ RT-PCR is a useful tool for the detection of ERalpha mRNA in selected cases, both in vitro and ex vivo. J Clin PATHOL: Mol Pathol

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research