Open AccessStability study of hydromorphone and bupivacaine mixture by HPLC-UV
Author(s) -
Charlotte Macorigh,
Vincent Guibbert,
Marine Casanova,
Catherine Haenni
Publication year - 2018
Publication title -
european journal of hospital pharmacy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.313
H-Index - 14
eISSN - 2047-9964
pISSN - 2047-9956
DOI - 10.1136/ejhpharm-2018-001553
Subject(s) - chromatography , high performance liquid chromatography , hydromorphone , chemistry , detection limit , bupivacaine , acetonitrile , particle size , chemical stability , analytical chemistry (journal) , anesthesia , medicine , organic chemistry , biochemistry , receptor , opioid
The objective of this study was to evaluate the physical and chemical stability of hydromorphone hydrochloride and bupivacaine hydrochloride in concentrations of 15 mg.ml -1 and 10 mg.mL -1 in 0.9% sodium chloride injection. Test samples of hydromorphone/bupivacaine mixtures were stored at 37°C, body temperature encounterd during continuous intrathecal infusion, for 90 days. The solutions were packaged in 20 ml plastic syringes. Evaluations for physical and chemical stability were performed initially and throughout the storage periods. Physical stability was assessed by visual observation. The chemical stability of the drug was evaluated by means of a stability-indicating high-performance liquid chromatographic (HPLC) analytical technique. In addition, pH and osmolarity were measured electronically.