Open AccessModified Giemsa method for confirmation of Pneumocystis pneumonia in low-income countries
Author(s) -
Alfred Andama,
Adithya Cattamanchi,
J. Lucian Davis,
Saskia den Boon,
William Worodria,
Laurence Huang
Publication year - 2009
Publication title -
bmj case reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.231
H-Index - 26
ISSN - 1757-790X
DOI - 10.1136/bcr.02.2009.1580
Subject(s) - medicine , giemsa stain , pneumocystis jirovecii , pulmonology , bronchoalveolar lavage , pneumocystis pneumonia , pneumonia , sputum , pneumocystis carinii , pathology , lung , tuberculosis
An HIV-seropositive man who presented with cough, low-grade fever and difficulty in breathing was admitted to the pulmonology unit at Mulago Hospital in Kampala, Uganda. He was initially diagnosed with bacterial pneumonia and treated with ceftriaxone without significant improvement. Induced sputum and bronchoalveolar lavage specimens were collected and stained using a modified Giemsa stain (Diff-Quik). With this technique, it was possible to demonstrate cystic and trophic forms of Pneumocystis jirovecii and confirm the diagnosis of Pneumocystis pneumonia in this patient.