Open AccessMutation of MtrA at the Predicted Phosphorylation Site Abrogates Its Role as a Global Regulator in Streptomyces venezuelae
Author(s) -
Ting Lu,
Yanping Zhu,
Nan Xue,
Xia Zhang,
Yang Liu,
Xiqing Cui,
Xiuhua Pang
Publication year - 2022
Publication title -
microbiology spectrum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.502
H-Index - 51
ISSN - 2165-0497
DOI - 10.1128/spectrum.02131-21
Subject(s) - regulator , mutation , phosphorylation , genetics , biology , gene
Although phosphorylation has been shown to be essential for the activation of many response regulator proteins of two-component systems, the role of the phosphorylation site in the function of the global regulator MtrA in the genusStreptomyces has not been reported. In this study, we generatedStreptomyces mutants that had amino acid substitutions at the predicted phosphorylation site of MtrA, and the effects of the substitutions were investigated by comparing the phenotypes of the resulting strains and their gene expression patterns with those of the wild-type strain and an MtrA deletion mutant.