Open AccessDsrA Modulates Central Carbon Metabolism and Redox Balance by Directly Repressing pflB Expression in Salmonella Typhimurium
Author(s) -
Rui Dong,
Yuan Liang,
Shoukui He,
Yan Cui,
Chunlei Shi,
Yiping He,
Xianming Shi
Publication year - 2022
Publication title -
microbiology spectrum
Language(s) - English
Resource type - Journals
ISSN - 2165-0497
DOI - 10.1128/spectrum.01522-21
Subject(s) - biology , rnase p , gene expression , genetics , gene , biochemistry , microbiology and biotechnology , rna
Small RNA DsrA plays an important role in defending against oxidative stress in bacteria. In this study, we identified a novel target (pflB , encoding pyruvate-formate lyase) of DsrA and demonstrated its potential regulatory mechanism inS. Typhimurium by transcriptome analysis.In silico prediction revealed a direct base pairing between DsrA andpflB mRNA, which was confirmed in site-directed mutagenesis experiments.