Open AccessDifferentiating Trypanosoma cruzi in a Host Mammalian Cell Imaged in Aqueous Liquid by Atmospheric Scanning Electron Microscopy
Author(s) -
Yuko Takagi,
Mari Sato,
Masami Naya,
Chikara Sato
Publication year - 2022
Publication title -
microbiology spectrum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.502
H-Index - 51
ISSN - 2165-0497
DOI - 10.1128/spectrum.01413-21
Subject(s) - trypanosoma cruzi , biology , intracellular , biophysics , microbiology and biotechnology , intracellular parasite , amastigote , scanning electron microscope , electron microscope , cell , parasite hosting , biochemistry , materials science , optics , physics , world wide web , computer science , composite material , leishmania
Using Atmospheric Scanning Electron Microscopy (ASEM), we visualized interaction between infectious stage ofTrypanosoma cruzi and completely intact host mammalian cell. Plasma membrane appears translucent under ASEM, which not only enables direct observation ofT. cruzi within its host cell, but also reveals internal structures of the parasite itself.